The Tox21 10K Compound Library: Collaborative Chemistry Advancing Toxicology
نویسندگان
چکیده
منابع مشابه
Profiling of the Tox21 10K compound library for agonists and antagonists of the estrogen receptor alpha signaling pathway
The U.S. Tox21 program has screened a library of approximately 10,000 (10K) environmental chemicals and drugs in three independent runs for estrogen receptor alpha (ERα) agonist and antagonist activity using two types of ER reporter gene cell lines, one with an endogenous full length ERα (ER-luc; BG1 cell line) and the other with a transfected partial receptor consisting of the ligand binding d...
متن کاملCell-Based High-Throughput Screening for Aromatase Inhibitors in the Tox21 10K Library.
Multiple mechanisms exist for endocrine disruption; one nonreceptor-mediated mechanism is via effects on aromatase, an enzyme critical for maintaining the normal in vivo balance of androgens and estrogens. We adapted the AroER tri-screen 96-well assay to 1536-well format to identify potential aromatase inhibitors (AIs) in the U.S. Tox21 10K compound library. In this assay, screening with compou...
متن کاملThe U.S. “Tox21 community” and the future of toxicology testing
In early 2008, the National Institute of environmental Health Sciences/National toxicology Program, the NIH Chemical Genomics Center, and the environmental Protection Agency’s National Center for Computational toxicology entered into a Memorandum of Understanding to collaborate on the research, development, validation and translation of new and innovative test methods that characterize key step...
متن کاملReal-time cell toxicity profiling of Tox21 10K compounds reveals cytotoxicity dependent toxicity pathway linkage
Cytotoxicity is a commonly used in vitro endpoint for evaluating chemical toxicity. In support of the U.S. Tox21 screening program, the cytotoxicity of ~10K chemicals was interrogated at 0, 8, 16, 24, 32, & 40 hours of exposure in a concentration dependent fashion in two cell lines (HEK293, HepG2) using two multiplexed, real-time assay technologies. One technology measures the metabolic activit...
متن کاملCorrection: Real-time cell toxicity profiling of Tox21 10K compounds reveals cytotoxicity dependent toxicity pathway linkage
[This corrects the article DOI: 10.1371/journal.pone.0177902.].
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ژورنال
عنوان ژورنال: Chemical Research in Toxicology
سال: 2020
ISSN: 0893-228X,1520-5010
DOI: 10.1021/acs.chemrestox.0c00264